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The growth in vitro of vaccinia virus in chick embryo chorio-allantoic memberane, minced embryo and cell suspensions

Published online by Cambridge University Press:  15 May 2009

H. B. Maitland  and
D. I. Magrath
H. B. Maitland
Affiliation:
Department of Bacteriology, University of Manchester
D. I. Magrath
Affiliation:
Department of Bacteriology, University of Manchester
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The growth curve of rabbit skin-adapted vaccinia virus in the chick chorioallantoic membrane incubated in Hanks' solution showed a drop in titre of virus for about 10 hr. followed by growth. At least 25% of virus, sometimes more, remained infective. A similar fall in titre was observed in heated membranes in which the virus did not grow and this occurred also when membranes, either normal or heated, were infected and disintegrated before incubation.

The growth curve of virus in minced chick-embryo was similar to that in chorioallantoic membrane.

Virus in cell suspensions prepared from chick embryo and incubated in a nutrient medium showed only a small loss of infectivity before growth in some experiments and rarely dropped below 65–70 % of the original titre in others.

These results throw considerable doubt on the view that loss of infectivity preceding growth of vaccinia virus should be interpreted as an essential part of a growth cycle.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 55 , Issue 3 , September 1957 , pp. 347 - 360
Copyright
Copyright © Cambridge University Press 1957

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