Abstract
A complete copy of the mRNA sequences encoding human coagulation factor VIII:C has been cloned and expressed. The DNA sequence predicts a single chain precursor of 2,351 amino acids with a relative molecular mass (Mr) 267,039. The protein has an obvious domain structure, contains sequence repeats and is structurally related to factor V and ceruloplasmin.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 52 print issues and online access
$199.00 per year
only $3.83 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Hoyer, L. W. Blood 58, 1–12 (1981).
Rapaport, S. I., Schiffman, S. & Patch, M. J. Blood 21, 221–235 (1963).
Vehar, G. A. & Davie, E. W. Biochemistry 19, 410–416 (1980).
Fass, D. N., Knutson, G. J. & Katzman, J. A. Blood 59, 594–600 (1982).
Fulcher, C. A., Gardiner, J. E., Griffin, J. H. & Zimmerman, T. A. Blood 63, 486–489 (1984).
Fass, D. N., Hewick, R. M., Knutson, G. J., Nesheim, M. E. & Mann, K. G. Proc. natn. Acad. Sci. U.S.A. (in the press).
Owen, C. A., Jr Bowie, W. E. J. & Fass, D. N. Br. J. Haemat. 43, 307–315 (1979).
Libre, E. P., Cowen, D. H., Watkins, S. P. & Shulman, N. R. Blood 31, 358–368 (1968).
Marchioro, T. L., Houghie, C., Ragde, H., Epstein, R. B. & Thomas, E. D. Science 163, 188–190 (1969).
Knutson, G. J. & Fass, D. N. Blood 59, 615–624 (1982).
Hewick, R. M., Hunkapiller, M. E., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990–7997 (1982).
Strehler, B. & North, D. Mech. Ageing Dev. 18, 285–313 (1982).
Agarwal, K. L., Brunstedt, J. & Noyes, B. E. J. biol. Chem. 256, 1023–1028 (1981).
Jaye, M. et al. Nucleic Acids Res. 11, 2325–2335 (1983).
Lawn, R. M., Fritsch, E. F., Parker, R. C., Blake, G. & Maniatis, T. Cell 15, 1157–1174 (1978).
Stel, H. V., vanderKwast, Th. H. & Veerman, E. C. I. Nature 303, 530–532 (1983).
Kaufman, R. J. & Sharp, P. A. Molec. Cell Biol. 2, 1304–1319 (1982).
Clark, S. C. et al. Proc. natn. Acad. Sci. U.S.A. 81, 2541–2547 (1984).
Hopp, T. P. & Woods, K. R. Proc. natn. Acad. Sci. U.S.A. 78, 3824–3828 (1981).
Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).
Church, W. R. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Takahashi, N., Ortel, T. L. & Putnam, F. W. Proc. natn. Acad. Sci. U.S.A. 81, 390–394 (1984).
Gluzman, Y. Cell 23, 175–182 (1981).
Sompayrac, L. M. & Dana, K. J. Proc. natn. Acad. Sci. U.S.A. 78, 7575–7578 (1981).
Luthman, H. & Magnusson, G. Nucleic Acids Res. 11, 1295–1308 (1983).
Lee, M. L., Maglalang, E. A. & Kingdon, H. S. Thromb. Res. 30, 511–519 (1983).
Kasper, C. K. Thromb. Diath. Haemorrh 34, 869–872 (1975).
Hunkapillar, M. W., Lujan, E., Ostrander, F. & Hood, L. E. Meth. Enzym. 91, 227–236 (1983).
Woo, S. L. C. Meth. Enzym. 68, 389–395 (1979).
Hastie, N. D., Held, W. & Toole, J. J. Cell 17, 449–457 (1979).
Derman, E. et al. Cell 23, 731–739 (1981).
O'Hare, K., Levis, R. & Rubin, G. M. Proc. natn. Acad. Sci. U.S.A. 80, 6917–6921 (1983).
Shaw, P. H., Held, W. A. & Hastie, N. D. Cell 32, 755–761 (1983).
Gubler, U. & Hoffman, B. J. Gene 25, 163–269 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Toole, J., Knopf, J., Wozney, J. et al. Molecular cloning of a cDNA encoding human antihaemophilic factor. Nature 312, 342–347 (1984). https://doi.org/10.1038/312342a0
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1038/312342a0
