Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer
- Iris J. M. Levink1
- Kateryna Nesteruk1
- Dido I. Visser1
- Anieta M. Sieuwerts3
- Celio J.C. Fernandes1
- Maurice P.H.M. Jansen2
- Lydi M.J.W. van Driel1
- Jan-Werner Poley1
- Maikel P. Peppelenbosch1
- Djuna L. Cahen1
- Gwenny M. Fuhler1
- Marco J. Bruno1
INTRODUCTION:
Imaging-based surveillance programs fail to detect pancreatic ductal adenocarcinoma at a curable stage, creating an urgent need for diagnostic biomarkers.
METHODS:
Secretin-stimulated pancreatic juice (PJ) was collected from the duodenal lumen during endoscopic ultrasound. The yield of biomarkers and organoids was compared for 2 collection techniques (endoscope suction channel vs catheter-based) and 3 periods (0–4 vs 4–8 vs 8–15 minutes).
RESULTS:
Collection through the endoscope suction channel was superior to collection with a catheter. Collection beyond 8 minutes reduced biomarker yield. PJ-derived organoid culture was feasible.
DISCUSSION:
The optimal protocol for secretin-stimulated PJ collection is through the endoscope suction channel for 8 minutes allowing biomarker detection and organoid culture.
