5'-3' exoribonuclease 2

XRN2
Identifiers
Aliases	XRN2, entrez:22803, 5'-3' exoribonuclease 2
External IDs	OMIM: 608851; MGI: 894687; GeneCards: XRN2
Gene location (Human) Chr. Chromosome 20 (human) Band 20p11.22 Start 21,303,331 bp End 21,389,825 bp
Gene location (Mouse) Chr. Chromosome 2 (mouse) Band 2|2 G2 Start 146,854,916 bp End 146,919,920 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in monocyte ganglionic eminence gonad islet of Langerhans smooth muscle tissue Achilles tendon appendix gallbladder ventricular zone stromal cell of endometrium Top expressed in tail of embryo yolk sac Gonadal ridge ventricular zone ganglionic eminence neural layer of retina mandibular prominence maxillary prominence abdominal wall spermatid More reference expression data BioGPS n/a
Gene ontology Molecular function DNA binding 5'-3' exoribonuclease activity 5'-3' exonuclease activity metal ion binding protein binding nucleic acid binding nuclease activity transcription termination site sequence-specific DNA binding exonuclease activity hydrolase activity 3'-5'-exoribonuclease activity RNA binding Cellular component membrane aggresome nucleolus nucleus nucleoplasm Biological process RNA processing regulation of transcription, DNA-templated DNA-templated transcription, termination mRNA processing RNA catabolic process transcription, DNA-templated DNA catabolic process, exonucleolytic spermatogenesis RNA metabolic process nucleobase-containing compound metabolic process neuron differentiation retina development in camera-type eye rRNA processing RNA phosphodiester bond hydrolysis, exonucleolytic hippocampus development Sources:Amigo / QuickGO
Orthologs Databases NCBI: entry; OMA: entry Species Human Mouse Entrez 22803 24128 Ensembl ENSG00000088930 ENSMUSG00000027433 UniProt Q9H0D6 Q9DBR1 RefSeq (mRNA) NM_012255 NM_001317960 NM_011917 NM_001356402 NM_001356403 RefSeq (protein) NP_001304889 NP_036387 NP_036047 NP_001343331 NP_001343332 Location (UCSC) Chr 20: 21.3 – 21.39 Mb Chr 2: 146.85 – 146.92 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

5'-3' Exoribonuclease 2 (XRN2) also known as Dhm1-like protein is an exoribonuclease enzyme that in humans is encoded by the XRN2 gene.

The human gene encoding XRN2 shares similarity with the mouse Dhm1 and the yeast's Dhp1 (Schizosaccharomyces pombe) or RAT1 (Saccharomyces) genes. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking and termination. Complementation studies show that Dhm1 has a similar function in mouse as Dhp1.

Function

Human XRN2 is involved in the torpedo model of transcription termination.

The C. elegans homologue, XRN-2, is involved in the degradation of certain mature miRNAs and their dislodging from miRISC miRNAs.

In yeast, the Rat1 protein has been shown to also be involved in the torpedo transcription termination model. When a polyadenylation site has been detected on the nascent RNA and cleaved by the RNA polymerase II, the Rtt103 factor recruits Rat1 and attaches it to free end. The exonuclease activity of Rat1 degrades the RNA strand and halts transcriptions upon catching up to the polymerase.

See also

• Xrn1

External links

• Human XRN2 genome location and XRN2 gene details page in the UCSC Genome Browser.

Further reading

• Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation". Genome Research. 14 (7): 1315–1323. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (October 2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nature Biotechnology. 24 (10): 1285–1292. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
• Lu Y, Liu P, James M, Vikis HG, Liu H, Wen W, et al. (February 2010). "Genetic variants cis-regulating Xrn2 expression contribute to the risk of spontaneous lung tumor". Oncogene. 29 (7): 1041–1049. doi:10.1038/onc.2009.396. PMC 6019621. PMID 19915612.
• Lejeune F, Li X, Maquat LE (2003). "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities". Molecular Cell. 12 (3): 675–687. doi:10.1016/S1097-2765(03)00349-6. PMID 14527413.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, et al. (March 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422–435. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Yang XC, Sullivan KD, Marzluff WF, Dominski Z (2009). "Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing". Molecular and Cellular Biology. 29 (1): 31–42. doi:10.1128/MCB.00776-08. PMC 2612496. PMID 18955505.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, et al. (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–648. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Scherl A, Couté Y, Déon C, Callé A, Kindbeiter K, Sanchez JC, et al. (November 2002). "Functional proteomic analysis of human nucleolus". Molecular Biology of the Cell. 13 (11): 4100–4109. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
• Kaneko S, Rozenblatt-Rosen O, Meyerson M, Manley JL (2007). "The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination". Genes & Development. 21 (14): 1779–1789. doi:10.1101/gad.1565207. PMC 1920172. PMID 17639083.
• Gromak N, West S, Proudfoot NJ (2006). "Pause sites promote transcriptional termination of mammalian RNA polymerase II". Molecular and Cellular Biology. 26 (10): 3986–3996. doi:10.1128/MCB.26.10.3986-3996.2006. PMC 1488997. PMID 16648491.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, et al. (January 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Li J, Zheng H, Ji C, Fei X, Zheng M, Gao Y, et al. (April 2005). "A novel splice variant of human XRN2 gene is mainly expressed in blood leukocyte". DNA Sequence. 16 (2): 143–146. doi:10.1080/10425170500066771. PMID 16147866. S2CID 35865401.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, et al. (August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–12135. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.

External links

• XRN2+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)