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Clinical Trial
. 2002 Aug 7:2:9.
doi: 10.1186/1471-5945-2-9.

Early inflammatory markers in elicitation of allergic contact dermatitis

Andrea Martín  1 , Norberto Gallino, Julio Gagliardi, Susana Ortiz, Alejandro Ruiz Lascano, Ana Diller, María Cristina Daraio, Adrián Kahn, Ana Lía Mariani, Horacio Marcelo Serra
Affiliations
Clinical Trial

Early inflammatory markers in elicitation of allergic contact dermatitis

Andrea Martín et al. BMC Dermatol. .

Abstract

Background: Allergic Contact Dermatitis (ACD) is regarded as a T-cell-mediated delayed-type hypersensitivity reaction. We studied the kinetics of the expression of CS-1 fibronectin, thymus and activation-regulated chemokine (CCL17/ TARC) and different chemokine receptors (CR) in skin biopsies from individuals suffering from back problems, with the antigen responsible of their contact dermatitis and an irrelevant antigen.

Methods: Samples were taken at 2, 10, and 48 hours for histological and immunohistochemical studies using monoclonal antibodies against human CS-1 fibronectin, CCL17, CD3, CD68, CD49d, CXCR3, CCR5, and CCR3.

Results: At positive antigen stimulated sites there was an early expression of CS-1 fibronectin (2 hours), followed by CCL17 and a later accumulation of alplha4/beta1+ (CD49d), CD3+, CD68+, CXCR3+ and CCR5+ mononuclear cells. At 48 hours, approximately 59 % of infiltrating cells were CXCR3+, 42% CCR5+, and only 14 % CCR3+.

Conclusions: These results showed for the first time a very early expression of CS-1 fibronectin which preceded production of CCL17 in blood endothelial cells (BCEs) from patients' skin with ACD. The role of these molecules in recruitment of monocytes and effector T cells in ACD is discussed.

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Figures

Figure 1
Figure 1
Micrograph of skin biopsies after specific Ag challenge at 2 h (A), 10 h (B) and 48 h (C) and negative Ag site at 48 h (D) stained with hematoxilin/eosin. Insert in upper right of each image show enlarged views of BECs from the same tissue section. Original magnifications × 10 and × 40
Figure 2
Figure 2
Micrograph of biopsies after specific Ag challenge at 2 h (A, E), 10 h (B, F) and 48 h (C, G), and negative Ag site at 48 h (D, H) stained for CS-fibronectin (A-D) and CCL17 (E-H). Original magnifications × 40.
Figure 3
Figure 3
Numbers of positive cells (mean +/- SEM) per square millimeter of α4β1+ (CD49d), CD3+ and CD68+ (A), CCR5+, CXCR3+ and CCR4+ (B) infiltrating cells in antigen-challenged skin biopsies. Negative antigen-challenged sites (Ag-) at 48 h were used as control. Significant differences (Ag- and 2-, 10- and 48-h specific Ag challenge time points; Friedman's test) were observed for CD3+ T lymphocytes (p < 0.0001), CD68+ macrophages (p < 0.0001), CXCR3+ cells (p < 0.003), CCR4+ cells (p < 0.003), and CCR3+ cells (p < 0.003).
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