The Analysis of Variance (ANOVA) test is a statistical method used to determine whether there are significant differences between the means of three or more groups.
Example 1: Three different kinds of food are tested on three groups of rats for 5 weeks. The objective is to check the difference in mean weight (in grams) of the rats per week. Apply one-way ANOVA using a 0.05 significance level to the following data:
| Food I | Food II | Food III |
|---|---|---|
| 8 | 4 | 11 |
| 12 | 5 | 8 |
| 19 | 4 | 7 |
| 8 | 6 | 13 |
| 6 | 9 | 7 |
| 11 | 7 | 9 |
Solution:
H0: μ1= μ2=μ3
H1: The means are not equalSince, X̄1 = 5, X̄2 = 9, X̄3 = 10
Total mean = X̄ = 8
- SSB = 6(5 - 8)2 + 6(9 - 8)2 + 6(10 - 8)2 = 84
- SSE = 68
- MSB = SSB/df1 = 42
- MSE = SSE/df2 = 4.53
- f = MSB/MSE = 42/4.53 = 9.33
Since f > F, the null hypothesis stands rejected.
Example 2: Calculate the ANOVA coefficient for the following data:
| Plant | Number | Average span | s |
|---|---|---|---|
| Hibiscus | 5 | 12 | 2 |
| Marigold | 5 | 16 | 1 |
| Rose | 5 | 20 | 4 |
Solution:
Plant n x s s2 Hibiscus 5 12 2 4 Marigold 5 16 1 1 Rose 5 20 4 16 p = 3
n = 5
N = 15
x̄ = 16SST = Σn(x−x̄)2
- SST= 5(12 − 16)2 + 5(16 − 16)2 + 11(20 − 16)2 = 160
- MST = SST/p-1 = 160/3-1 = 80
- SSE = ∑ (n−1) = 4 (4 + 1) + 4(16) = 84
- MSE = 7
- F = MST/MSE = 80/7
- F = 11.429
Example 3: The following data show the number of worms quarantined from the GI areas of four groups of muskrats in a carbon tetrachloride anthelmintic study. Conduct a two-way ANOVA test.
| I | II | III | IV |
|---|---|---|---|
| 338 | 412 | 124 | 389 |
| 324 | 387 | 353 | 432 |
| 268 | 400 | 469 | 255 |
| 147 | 233 | 222 | 133 |
| 309 | 212 | 111 | 265 |
Solution:
Source of Variation Sum of Squares Degrees of Freedom Mean Square Between the groups 62111.6 8 9078.067 Within the groups 98787.8 16 4567.89 Total 167771.4 24 Since F = MST / MSE
= 9.4062 / 3.66
F = 2.57
Example 4: Enlist the results in APA format after performing ANOVA on the following data set:
Solution:
- Variance of first set = (10.45)2 = 109.2
- Variance of second set = (12.76)2 = 162.82
- Variance of third set = (11.47)2 = 131.56
MSerror = {109.2 + 162.82 + 131.56} / {3}
= 134.53MSbetween = (17.62)(30) = 528.75
F = MSbetween / MSerror
= 528.75 / 134.53
F = 4.86APA writeup: F(2, 87)=3.93, p >=0.01, η2=0.08.
Practice Problem
Question 1. Method A = {80, 85, 90, 87}, Method B = {75, 78, 72, 74}, and Method C = {88, 85, 90, 92} are given. State the null and alternative hypotheses for performing a One-Way ANOVA test.
Question 2. Calculate the F-statistic for the given data using One-Way ANOVA. Group 1 = {5, 6, 7, 8}, Group 2 = {4, 5, 6, 5}, and Group 3 = {7, 7, 6, 8}.
Question 3. Interpret the significance of the p-value for the interaction effect in a Two-Way ANOVA, where the p-value for the interaction effect is 0.02 and the significance level (α) is 0.05.
Question 4. Group A = {10, 12, 14, 13}, Group B = {15, 17, 16, 18}, and Group C = {20, 22, 21, 23} are given. Interpret the p-value of the ANOVA test and explain whether the null hypothesis is rejected, where F-statistic = 4.86 and p-value = 0.01.
Answer:-
- Null Hypothesis (H₀): μ₁ = μ₂ = μ₃ (The means of all groups are equal). Alternative Hypothesis (H₁): At least one mean is different.
- F-statistic = 4.58.
- If the p-value (0.02) is less than the significance level (0.05), reject the null hypothesis and conclude that there is a significant interaction effect.
- Since the p-value (0.01) is less than 0.05 and the F-statistic is significant, we reject the null hypothesis, indicating a significant difference between the group means.